Open AccessIdentification of PER‐1 extended‐spectrum β‐lactamase producing Pseudomonas aeruginosa clinical isolates of the international clonal complex CC11 from Hungary and Serbia
Author(s) -
Libisch Balázs,
Poirel Laurent,
Lepsanovic Zorica,
Mirovic Veljko,
Balogh Boglárka,
Pászti Judit,
Hunyadi Zsuzsanna,
Dobák András,
Füzi Miklós,
Nordmann Patrice
Publication year - 2008
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00483.x
Subject(s) - pseudomonas aeruginosa , integron , multilocus sequence typing , biology , microbiology and biotechnology , pseudomonadaceae , gene , genetics , bacteria , antibiotics , antibiotic resistance , genotype
PER‐1 extended‐spectrum β‐lactamase‐producing Pseudomonas aeruginosa clinical isolates from Budapest, Hungary, and Belgrade, Serbia, were characterized by molecular methods. Two PER‐1‐positive isolates were recovered from sporadic cases in Budapest and a small cluster of PER‐1‐positive infections involving four patients were identified at a Belgrade hospital. A class 1 integron harbouring a bla OXA‐2 β‐lactamase gene and four other gene cassettes was detected in both the Budapest and the Belgrade isolates. The two P. aeruginosa isolates from Budapest also carried another class 1 integron containing bla OXA‐74 , aac ( 6 ′) ‐Ib‐cr and cmlA7 genes in its variable region. The aac ( 6 ′)‐ Ib‐cr fluoroquinolone‐acetylating aminoglycoside acetyltransferase gene is described here for the first time in P. aeruginosa . Multilocus sequence typing (MLST) revealed that the PER‐1 positive P. aeruginosa isolates identified in this study display ST235, a sequence type that belongs to clonal complex CC11. Two bla PER‐1 ‐positive P. aeruginosa reference isolates from France and Belgium could also be assigned to complex CC11 by MLST. Our results underscore the role of complex CC11 in the dissemination of bla PER‐1 among P. aeruginosa clinical isolates.