Open AccessImmune responsiveness of a novel peptidoglycan conjugate prepared from surface Candida immunogens: mannan and CR3‐related protein
Author(s) -
Paulovičová Ema,
Bystrický Slavomír,
Machová Eva,
Bujdáková Helena
Publication year - 2008
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00442.x
Subject(s) - glycoconjugate , mannan , biology , immune system , ic3b , glycoprotein , antigen , microbiology and biotechnology , complement system , immunology , biochemistry , polysaccharide
Candidaalbicans expresses a CR3‐related protein (CR3‐RP) antigenically, structurally and functionally related to human adhesion glycoprotein, also known as Mac‐l, the iC3b receptor, or complement receptor type 3. The purpose of the present study was to analyze the immunogenic properties of a novel CR3‐RP glycoconjugate in a rabbit model. Cell‐mediated responses revealed immunoenhancement triggered by CR3‐RP glycoconjugate with respect to expression of IL‐2 receptor subunit CD25 on B‐lymphocytes and inductive increase of the CD4 + /CD8 + ratio compared with unconjugated cell wall mannan ( P <0.001). Active immunization with the CR3‐RP glycoconjugate resulted in an effective IgM–IgG isotypic switch even after the second booster. Altogether, it could be assumed that the novel peptide glycoconjugate is a prospective antigenic candidate for further Candida vaccine design.