Open AccessEnhanced resistance against systemic Candida albicans infection in mice treated with C. albicans DNA
Author(s) -
Dimitrova Petya,
Yordanov Martin,
Danova Svetla,
Ivanovska Nina
Publication year - 2008
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00421.x
Subject(s) - candida albicans , biology , microbiology and biotechnology , dna , corpus albicans , immunology , virology , genetics
Abstract In this study, double‐stranded Candida albicans DNA was administered in systemic C. albicans infection in at dose of 20 μg per mouse at 4, 5 and 6 weeks of age. The level of IL‐12 in serum was elevated as a result of yeast DNA treatment and correlated with lower mortality and decreased kidney and liver injury. Macrophage activation was demonstrated by an increase of nitric oxide (NO) and IL‐12 production. These effects were Janus activation kinases (JAK)/signal transducer and activator of transcription (STAT) dependent as they were inhibited by selective JAK inhibitor tyrphostin AG‐490. DNA influenced adaptive immune response through elevation of anti‐ Candida IgG antibody production in systemic C. albicans infection. Thus, C. albicans DNA augmented innate and adaptive immune responses against the pathogen.