Open AccessEffect of the HP0159 ORF mutation on the lipopolysaccharide structure and colonizing ability of Helicobacter pylori
Author(s) -
Altman Eleonora,
Chandan Vandana,
Larocque Suzon,
Aubry Annie,
Logan Susan M.,
Vinogradov Evgeny,
Li Jianjun
Publication year - 2008
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00416.x
Subject(s) - lipopolysaccharide , helicobacter pylori , mutant , heptose , biology , microbiology and biotechnology , gene , genetics , immunology
The outer core region of Helicobacter pylori lipopolysaccharide of the majority of isolates contains an α‐1,6‐glucan polymer synthesized by the product of the HP0159 ORF. Structural studies carried out on HP0159 lipopolysaccharide mutants by a combination of chemical methods, mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that insertional inactivation of HP0159 gene in H. pylori strains 26695 and SS1 resulted in formation of a truncated lipopolysaccharide molecule characterized by the presence of a terminal dd ‐heptose residue in the side‐chain outer core fragment and maintaining an inner core backbone structure compared with the wild‐type Lewis antigen‐expressing strains. Colonization studies with HP0159 mutants of two mouse‐colonizing strains, SS1 and M6, confirmed their inability to successfully colonize the murine stomach.