Open AccessStructure of a phosphoethanolamine‐containing O‐polysaccharide of Citrobacter freundii strain PCM 1443 from serogroup O39 and its relatedness to the Klebsiella pneumoniae O1 polysaccharide
Author(s) -
Katzenellenbogen Ewa,
Toukach Philip V.,
Kocharova Nina A.,
KorzeniowskaKowal Agnieszka,
Gamian Andrzej,
Shashkov Alexander S.,
Knirel Yuriy A.
Publication year - 2008
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00396.x
Subject(s) - citrobacter freundii , polysaccharide , klebsiella pneumoniae , microbiology and biotechnology , lipopolysaccharide , galactose , biochemistry , biology , strain (injury) , galactan , disaccharide , chemistry , escherichia coli , anatomy , gene , endocrinology
Lipopolysaccharide was extracted from cells of Citrobacter freundii PCM 1443 from serogroup O39 and degraded by mild acid hydrolysis to give an O‐polysaccharide. Based on enzymatic and methylation analyses, along with 1 H and 13 C nuclear magnetic resonance spectroscopy, it was found that the lipopolysaccharide studied has two different linear polysaccharide chains of d ‐galactan type containing 3‐substituted galactose residues. One of the galactans has the disaccharide repeating units of α‐ d ‐galactopyranose and β‐ d ‐galactofuranose and the other is comprised of α‐ d ‐galactopyranose and β‐ d ‐galactopyranose, the latter being substituted in 25% repeats with P EtN at O‐6. An immunoblotting assay demonstrated that the lipopolysaccharide of C. freundii PCM 1443 is serologically related to that of Klebsiella pneumoniae O1, which contains the same galactan chains but is devoid of phosphoethanolamine.