Open AccessIntranasal immunization of mutant toxic shock syndrome toxin 1 elicits systemic and mucosal immune response against Staphylococcus aureus infection
Author(s) -
Narita Kouji,
Hu DongLiang,
Tsuji Takao,
Nakane Akio
Publication year - 2008
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2008.00384.x
Subject(s) - staphylococcus aureus , toxic shock syndrome , microbiology and biotechnology , biology , nasal administration , antibody , staphylococcal infections , immune system , immunology , immunization , mucous membrane of nose , toxin , spleen , bacteria , genetics
We investigated whether an intranasal immunization with mutant toxic shock syndrome toxin 1 (TSST‐1) could elicit a protective effect against nasal colonization as well as systemic infection of Staphyloccoccus aureus in a mouse model. Anti‐TSST‐1 antibody production in the mucosal exudates and in sera was efficiently induced. Bacterial numbers were reduced in spleen, liver and also nasal cavities in the early stage of nasal colonization, and the survival rate was significantly improved in the immunized mice. It was suggested that the neutralizing activity of antibodies and the enhanced bactericidal activity of neutrophils were involved in the protection against systemic S. aureus infection, and the secreted antibodies could be involved in reduction of S. aureu s bacterial counts in the nasal cavity.