Open AccessEffect of tumor necrosis factor‐α on intracellular Staphylococcus aureus in vascular endothelial cells
Author(s) -
Park Wan Beom,
Lee Jae Hoon,
Jeon Jae Hyun,
Lee Su Jin,
Kim Sung Han,
Kim Nam Joong,
Kim Hong Bin,
Oh Myoungdon,
Choe Kang Won
Publication year - 2008
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2007.00365.x
Subject(s) - staphylococcus aureus , intracellular , tumor necrosis factor alpha , umbilical vein , microbiology and biotechnology , biology , apoptosis , endothelial stem cell , necrosis , internalization , intracellular parasite , immunology , cell , bacteria , biochemistry , in vitro , genetics
Although tumor necrosis factor‐α (TNF‐α) is an important host factor against intracellular bacteria, little is known about the effect of TNF‐α on the persistence of intracellular Staphylococcus aureus in vascular endothelial cells. It was investigated whether recombinant human TNF‐α influences the survival of intracellular S. aureus (ATCC 29213) in human umbilical vein endothelial cells (HUVEC) under a condition with an antistaphylococcal agent, and its mechanism. The HUVECs were incubated with TNF‐α, oxacillin, or both in 24‐well plates for up to 48 h following internalization of S. aureus (10 6 CFU well −1 ) into HUVECs for 1 h. TNF‐α (1 ng mL −1 ) significantly reduced the number of intracellular S. aureus in HUVECs, and TNF‐α plus oxacillin eliminated more intracellular S. aureus in HUVEC than oxacillin alone. The LDH viability assay and quantification of apoptosis using photometric enzyme‐immunoassay showed that TNF‐α preferentially induced cell death and apoptosis of HUVECs infected with S. aureus compared with noninfected HUVECs. These results indicate that TNF‐α helps antistaphylococcal antibiotics to eliminate intracellular S. aureus in vascular endothelial cells, partly because TNF‐α preferentially induces apoptosis of endothelial cells infected by S. aureus .