Open AccessBasis of beneficial immunomodulation by monoclonal antibodies against Streptococcus mutans adhesin P1
Author(s) -
Isoda Ryutaro,
Robinette Rebekah A.,
Pinder Trina L.,
McArthur William P.,
Brady L. Jeannine
Publication year - 2007
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2007.00279.x
Subject(s) - monoclonal antibody , bacterial adhesin , epitope , streptococcus mutans , biology , microbiology and biotechnology , immune system , antibody , immunology , opsonin , humoral immunity , bacteria , escherichia coli , biochemistry , genetics , gene
We previously identified five monoclonal antibodies (MAbs) against Streptococcus mutans adhesin P1 that modulate the humoral response when bound to whole bacteria and immune complexes (ICs) are administered to BALB/c mice. The two MAbs that redirected the response towards increased efficacy recognize discontinuous epitopes involving pre‐alanine‐rich domain sequence; therefore, to evaluate whether epitope specificity contributes to a desirable outcome a further MAb with this characteristic was tested. A beneficial immune response was promoted. None of the three MAbs that promoted a beneficial response was opsonic, suggesting that increased uptake of ICs by phagocytes does not mediate the improvement of the IC‐elicited antibodies to inhibit bacterial adherence. Finally, two of the six anti‐P1 MAbs activated complement but did not partition according to desirable vs. nondesirable effects.