Open AccessThe transcriptional profile of Paracoccidioides brasiliensis yeast cells is influenced by human plasma
Author(s) -
Bailão Alexandre Melo,
Shrank Augusto,
Borges Clayton Luiz,
Parente Juliana Alves,
Dutra Valéria,
Felipe Maria Sueli Soares,
Fiúza Rogério Bento,
Pereira Maristela,
Soares Célia Maria de Almeida
Publication year - 2007
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2007.00277.x
Subject(s) - paracoccidioides brasiliensis , biology , microbiology and biotechnology , yeast , paracoccidioidomycosis , paracoccidioides , fungus , biochemistry , botany
Paracoccidioides brasiliensis causes infection through host inhalation of airborne propagules of the mycelial phase of the fungus, which reach the lungs, and then disseminate to virtually all parts of the human body. Here we describe the identification of differentially expressed genes in P. brasiliensis yeast cells, by analyzing cDNA populations from the fungus treated with human plasma, mimicking superficial infection sites with inflammation. Our analysis identified transcripts that are differentially represented. The transcripts upregulated in yeast cells during incubation in human plasma were predominantly related to fatty acid degradation, protein synthesis, sensing of osmolarity changes, cell wall remodeling and cell defense. The expression pattern of genes was independently confirmed.