Open AccessProduction of recombinant gG‐1 protein of herpes simplex virus type 1 in a prokaryotic system in order to develop a type‐specific enzyme‐linked immunosorbent assay kit
Author(s) -
Zandi Keivan,
Roostaee Mohammad Hassan,
Sadeghizadeh Majid,
Rasaee Mohammad Javad,
Sajedi Reza Hassan,
Soleimanjahi Hoorieh
Publication year - 2007
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2007.00247.x
Subject(s) - herpes simplex virus , biology , virology , alphaherpesvirinae , monoclonal antibody , virus , recombinant dna , serology , antibody , glycoprotein , herpesviridae , simplexvirus , blot , plasmid , microbiology and biotechnology , viral disease , immunology , gene , biochemistry
The herpes simplex viruses are important causes of disease worldwide. Herpes simplex virus type 1 (HSV‐1) is the primary cause of oral‐facial and pharyngeal infections and may cause herpetic whitlow, eye infections as well as severe and sometimes dangerous infections of the eyes and brain. HSV‐1 also accounts for 10–15% of all genital herpetic infections. Therefore, laboratory diagnosis of this virus and development of diagnostic serological techniques for HSV‐1 is of particular importance. In the present study, pTrc His2A‐gG1 plasmid, containing the full‐length glycoprotein G (gG) protein, was produced in a prokaryotic system for the first time. Upon confirmation of a 37‐kDa gG‐1 protein production in a prokaryotic system based on western blotting and monoclonal antibodies, the protein was produced at a large scale and purified by ion‐exchange chromatography using DEAE‐sepharose. An HSV‐1 type‐specific diagnostic kit was designed and developed and the specificity and sensitivity of this kit were demonstrated to be 89.5% and 100%, respectively, as compared with a commercially available kit. A significant correlation was shown between the developed kit and the commercial kit.