Open AccessNucleotide sequence of the gyrA gene of Arcobacter species and characterization of human ciprofloxacin‐resistant clinical isolates
Author(s) -
Abdelbaqi Khalil,
Ménard Armelle,
ProuzetMauleon Valérie,
Bringaud Frédéric,
Lehours Philippe,
Mégraud Francis
Publication year - 2007
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2006.00208.x
Subject(s) - arcobacter , biology , campylobacter , microbiology and biotechnology , nucleic acid sequence , genetics , phylogenetic tree , ciprofloxacin , sequence analysis , multilocus sequence typing , gene , bacteria , genotype , antibiotics
The nucleotide sequence of the gyrA gene of Arcobacter butzleri, Arcobacter cryaerophilus , Arcobacter cibarius , and Arcobacter skirrowii was determined. The deduced GyrA proteins are closely related to those of Wolinella succinogenes and Helicobacter pullorum , whereas those of Campylobacter species showed less sequence identity. The phylogenetic analysis of GyrA sequences provides a result similar to 16S rRNA gene sequence phylogenetic analysis and allows the discrimination among A. butzleri species. In addition, a Thr→Ile mutation at amino acid 85 in the quinolone resistance‐determining region was associated with ciprofloxacin resistance for two A. butzleri and one A. cryaerophilus ciprofloxacin‐resistant strains.