Open AccessTrehalose‐6‐phosphate Phosphatase from Mycobacterium tuberculosis induces humoral and cellular immune responses
Author(s) -
Zhang Min,
Yang Yanping,
Xu Ying,
Qie Yaqing,
Wang Jiuling,
Zhu Bingdong,
Wang Qingzhong,
Jin Ruiliang,
Xu Shengfeng,
Wang Honghai
Publication year - 2007
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2006.00174.x
Subject(s) - biology , immune system , microbiology and biotechnology , mycobacterium tuberculosis , trehalose , humoral immunity , cellular immunity , immunology , tuberculosis , biochemistry , medicine , pathology
Trehalose‐6‐phosphate phosphatase is an enzyme strictly essential for the growth of mycobacteria. Subcellular fractionation of Mycobacterium tuberculosis and M. bovis bacillus Calmette‐Guérin (BCG) located the trehalose‐6‐phosphate phosphatase in the cell wall and membrane fractions. Trehalose‐6‐phosphate phosphatase induced an increased Th1‐type immune response in mice, characterized by an elevated level of interferon‐γ in antigen‐stimulated splenocyte culture and a strong IgG2a antibody response. The trehalose‐6‐phosphate phosphatase was recognized by the sera of tuberculosis patients and BCG‐vaccinated donors. The mycobacterial trehalose‐6‐phosphate phosphatase is an immunodominant antigen, and it may be a candidate for vaccine development for the control of tuberculosis.