Open AccessComparative PCR‐based restriction fragment length polymorphism analysis of the plasmid gene orf3 of Chlamydia trachomatis and Chlamydia psittaci
Author(s) -
Storni Elisa,
Donati Manuela,
Marangoni Antonella,
Accardo Silvia,
Cevenini Roberto
Publication year - 2006
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2006.00149.x
Subject(s) - chlamydia psittaci , biology , psittacosis , chlamydia trachomatis , plasmid , restriction fragment length polymorphism , chlamydiaceae , recombinant dna , virology , restriction enzyme , gene , genetics , chlamydophila , polymerase chain reaction , chlamydia
The Bf aI digestion of PCR‐based restriction fragment length polymorphism analysis of the plasmid orf3 of Chlamydia trachomatis and Chlamydia psittaci provided evidence for two distinct restriction patterns, respectively. The nucleotide sequences of orf3 genes confirmed these differences. Serum antibodies against recombinant C. psittaci protein (pgp3) encoded by orf3 were detected both in pigeons with C. psittaci infection and in a human patient with psittacosis.