Open AccessSystemic and mucosal immunity to respiratory syncytial virus induced by recombinant Streptococcus gordonii surface‐displaying a domain of viral glycoprotein G
Author(s) -
Falcone Valeria,
Mihm Dagmar,
NeumannHaefelin Dieter,
Costa Caterina,
Nguyen Thien,
Pozzi Gianni,
Ricci Susanna
Publication year - 2006
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2006.00130.x
Subject(s) - streptococcus gordonii , biology , virology , virus , microbiology and biotechnology , antibody , immunization , immunity , recombinant dna , glycoprotein , mononegavirales , humoral immunity , paramyxoviridae , immunology , immune system , streptococcaceae , viral disease , gene , antibiotics , biochemistry
A conserved fragment comprising amino acid residues 130–230 of the G glycoprotein of human respiratory syncytial virus subtype A was expressed in the commensal bacterium Streptococcus gordonii . Recombinant streptococci displaying the G domain at the cell surface were used to immunize mice via both parenteral and mucosal routes. Subcutaneous immunization induced respiratory syncytial virus‐specific serum immunoglobin G (IgG) capable of partially controlling virus replication in the lungs. Intranasal immunization with live bacteria stimulated the production of IgA against both the whole virus and the G domain in serum and bronchoalveolar fluid. Upon challenge, immunized animals had significantly lower virus titres in the lungs than the controls. Our results show for the first time that the G domain‐expressing S. gordonii strain elicits both systemic and mucosal immunity that reduced respiratory syncytial virus replication in the lungs of mice.