Open AccessCytokine profiles in tuberculosis patients and healthy subjects in response to complex and single antigens of Mycobacterium tuberculosis
Author(s) -
AlAttiyah Raja'a,
Madi Nada M.,
ElShamy Abdulsalaam M.,
Wiker Harald G.,
Andersen Peter,
Mustafa Abu S.
Publication year - 2006
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2006.00110.x
Subject(s) - antigen , peripheral blood mononuclear cell , mycobacterium tuberculosis , biology , groel , interferon gamma , mycobacterium bovis , immunology , secretion , cytokine , microbiology and biotechnology , tumor necrosis factor alpha , tuberculosis , medicine , endocrinology , in vitro , pathology , biochemistry , escherichia coli , gene
Peripheral blood mononuclear cells (PBMC) were obtained from tuberculosis (TB) patients and Mycobacterium bovis bacillus Calmette–Guerin vaccinated healthy subjects. PBMC were tested for secretion of tumor necrosis factor‐α (TNF‐α), interferon‐γ (IFN‐γ), interleukin‐5 (IL‐5) and IL‐10 in response to complex (whole cells, culture filtrate and cell walls), single secreted (Ag85B, ESAT6, MPT64, PstS and MPT70) and single cytosolic (DnaK, GroES and GroEL) antigens of Mycobacterium tuberculosis . In the absence of antigens, detectable concentrations of TNF‐α, IFN‐γ and IL‐10 were secreted by PBMC of both donor groups, but the concentrations of only IL‐10 were significantly higher ( P =0.015) in TB patients than in healthy subjects. In the presence of complex antigens, PBMC secreted IFN‐γ and TNF‐α in response to all three preparations, whereas IL‐10 was secreted in response to whole cells and cell walls only. In the presence of single antigens, IFN‐γ was secreted in response to Ag85B, ESAT6 and MPT64 in TB patients and ESAT6 in healthy donors. Except for GroEL and DnaK, single antigens did not induce TNF‐α and IL‐10 secretion from PBMC in either donor group. The secretion of IFN‐γ, but not IL‐10, in the presence of Ag85B, ESAT6 and MPT64 supports their potential as subunit vaccine candidates against TB.