Open AccessEfficient intracellular multiplication of Legionella pneumophila in human monocytes requires functional host cell L‐type calcium channels
Author(s) -
Wieland Hagen,
Hechtel Nicole,
Faigle Marion,
Neumeister Birgid
Publication year - 2006
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2006.00092.x
Subject(s) - legionella pneumophila , intracellular , biology , genistein , tyrosine kinase , microbiology and biotechnology , calcium in biology , intracellular parasite , signal transduction , bacteria , genetics
The infectious agent of Legionnaires' disease, Legionella pneumophila , multiplies intracellularly in a variety of eukaryotic cells. Genistein, a tyrosine kinase inhibitor, has been shown to block intracellular replication of L. pneumophila without harming the infected host cell. The present study has been performed to investigate the underlying mechanism. We demonstrate that inhibition of intracellular bacterial growth by genistein is not mediated by its protein tyrosine kinase‐modulating effect but by inhibition of L‐type calcium channels of the infected host cell.