Open AccessThe effect of DNA priming‐protein boosting on enhancing humoral immunity and protecting mice against lethal HSV infections
Author(s) -
Soleimanjahi Hoorieh,
Roostaee Mohammad Hassan,
Rasaee Mohammad Javad,
Mahboudi Fereidoon,
Kazemnejad Anooshirvan,
Bamdad Taravat,
Zandi Keivan
Publication year - 2006
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2005.00015.x
Subject(s) - biology , virology , recombinant dna , dna vaccination , herpes simplex virus , immune system , virus , humoral immunity , antigen , antibody , glycoprotein , recombinant virus , immunization , vaccination , priming (agriculture) , microbiology and biotechnology , immunology , gene , biochemistry , botany , germination
Herpes simplex virus produces primary and latent infections with periodic recurrency. The prime‐boost immunization strategies were studied using a DNA vaccine carrying the full‐length glycoprotein D‐1 gene and a baculovirus‐derived recombinant glycoprotein D, both expressing herpes simplex virus glycoprotein D‐1 protein. Immunization with recombinant DNAs encoding antigenic proteins could induce cellular and humoral responses by providing antigen expression in vivo . Higher immune response, however, occurred when the recombinant proteins followed DNA inoculation. While all groups of the immunized mice and positive control group could resist virus challenge, a higher virus neutralizing antibody level was detected in the animals receiving recombinant protein following DNA vaccination.