Open AccessStructures of the core oligosaccharide and O‐units in the R‐ and SR‐type lipopolysaccharides of reference strains of Pseudomonas aeruginosa O‐serogroups
Author(s) -
Bystrova Olga V.,
Knirel Yuriy A.,
Lindner Buko,
Kocharova Nina A.,
Kondakova An.,
Zähringer Ulrich,
Pier Gerald B.
Publication year - 2006
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2005.00004.x
Subject(s) - pseudomonas aeruginosa , lipopolysaccharide , microbiology and biotechnology , oligosaccharide , biology , bacteria , mass spectrometry , pseudomonadaceae , antigen , electrospray ionization , nuclear magnetic resonance spectroscopy , strain (injury) , lipid a , pseudomonadales , chemistry , biochemistry , stereochemistry , chromatography , genetics , anatomy , endocrinology
Highly phosphorylated core oligosaccharides and those substituted with one O‐antigen repeating unit were obtained by mild acid degradation or strong alkaline hydrolysis of lipopolysaccharide samples from 23 reference strains representing all Pseudomonas aeruginosa O‐serogroups. Studies by high‐resolution electrospray ionization mass spectrometry and two‐dimensional NMR spectroscopy revealed both conserved and variable structural features of the lipopolysaccharides of various O‐serogroups. The upstream terminal saccharide of the O‐antigen, which contributes most to the immunospecificity of the bacteria, was defined in 11 from a total of 13 O‐serogroups. The data obtained link together the known biosynthesis pathways, genetics and serology of the P. aeruginosa lipopolysaccharide.