Open AccessThe partially degraded lipopolysaccharide of Burkholderia cepacia and ornithine‐containing lipids derived from some Gram‐negative bacteria are useful complex lipid adjuvants
Author(s) -
Kawai Yohko,
Watanabe Mineo,
Matsuura Motohiro,
Nishijima Masahiro,
Kawahara Kazuyoshi
Publication year - 2002
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2002.tb00621.x
Subject(s) - toxoid , lipid a , adjuvant , microbiology and biotechnology , lipopolysaccharide , biology , diphtheria toxin , burkholderia , clostridium butyricum , ornithine , immunization , antigen , bacteria , biochemistry , toxin , immunology , amino acid , genetics , arginine
The partially degraded lipopolysaccharide of Burkholderia cepacia (LPSdegr) and the ornithine‐containing lipids were purified from some bacteria. The substances were developed as complex lipid adjuvants, because they have weak toxicity and are able to activate the immune systems of the living body. After various toxoid antigens such as pertussis toxoid, diphtheria toxoid and tetanus toxoid were mixed with the complex lipid adjuvants, the mixtures were administered to mice subcutaneously. Antitoxoid IgG antibody titers in the serum were measured several times over 3 months. The efficacy of the LPSdegr as adjuvant was almost as high as that of the ornithine‐containing lipids, and it was almost equal to that of the aluminum hydroxide adjuvant (Alum), which is generally used as a vaccine adjuvant.