Open AccessEvaluation of efficacy in terms of antibody levels and cell‐mediated immunity of acellular pertussis vaccines in a murine model of respiratory infection
Author(s) -
Watanabe Mineo,
Komatsu Eiji,
Sato Takaaki,
Nagai Masaaki
Publication year - 2002
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2002.tb00594.x
Subject(s) - biology , pertussis toxin , pertactin , antibody , immunology , immune system , hemagglutinin (influenza) , microbiology and biotechnology , immunity , filamentous haemagglutinin adhesin , interferon gamma , pertussis vaccine , vaccination , virology , immunization , receptor , biochemistry , g protein
The efficacy of six acellular pertussis vaccines, prepared by various manufacturers in Japan, was investigated in a murine model of respiratory infection (aerosol challenge model) and a murine intracerebral (i.c.) challenge model. There was a good correlation between bacterial clearance from the lungs after aerosol challenge and the potency of vaccines as determined by i.c. challenge. The levels of antibodies against filamentous hemagglutinin were higher after immunizations with all tested vaccines than the levels of antibodies against pertussis toxin and pertactin. Spleen cells from mice immunized with each individual vaccine secreted interferon γ (IFN‐γ) in response to stimulation by pertussis toxin, filamentous hemagglutinin and fimbriae. The production of interleukin‐4 in response to each of the antigens tested was detected but was lower than that of IFN‐γ. However, antibody levels and cell‐mediated immune responses were not correlated with the protective effects of the vaccines after aerosol challenge and after i.c. challenge.