Open AccessEvaluation of DNA polymorphisms amplified by arbitrary primers (RAPD) as genetically associated elements to differentiate virulent and non‐virulent Paracoccidioides brasiliensis isolates
Author(s) -
Motta Teresa R,
MoreiraFilho Carlos A,
Mendes Rinaldo P,
Souza Lenice R,
Sugizak Maria F,
Baueb Sonia,
Calich Vera L.G,
Vaz Celideia A.C
Publication year - 2002
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2002.tb00585.x
Subject(s) - paracoccidioidomycosis , virulence , paracoccidioides brasiliensis , rapd , biology , paracoccidioides , microbiology and biotechnology , genotype , genetics , gene , genetic diversity , medicine , population , environmental health
Randomly amplified polymorphic DNA (RAPD) analysis of 35 Paracoccidioides brasiliensis isolates was carried out to evaluate the correlation of RAPD profiles with the virulence degree or the type of the clinical manifestations of human paracoccidioidomycosis. The dendrogram presented two main groups sharing 64% genetic similarity. Group A included two isolates from patients with chronic paracoccidioidomycosis; group B comprised the following isolates showing 65% similarity: two non‐virulent, six attenuated, five virulent, eight from patients with chronic paracoccidioidomycosis and two from patients with acute paracoccidioidomycosis. The virulent Pb18 isolate and six attenuated or non‐virulent samples derived from it were genetically indistinguishable (100% of similarity). Thus, in our study, RAPD patterns could not discriminate among 35 P. brasiliensis isolates according to their differences either in the degree of virulence or in the type of the clinical manifestation of this fungal infection.