Open AccessImmunogenic protein variations of Clostridium chauvoei cellular antigens associated with the culture growth phase
Author(s) -
Mattar María Aída,
Cortiñas Teresa Inés,
Guzmán Ana María Stefanini
Publication year - 2002
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2002.tb00565.x
Subject(s) - immunogenicity , biology , strain (injury) , microbiology and biotechnology , stationary phase , antigen , immunology , chemistry , chromatography , anatomy
The immunoprotective capacity of four Clostridium chauvoei strains at different growth stages is reported. In all the strains tested, the cells coming from the stationary phase were those with the highest immunoprotective capacity and, depending on the strain, this protective capacity diminished or even disappeared in other phases. Protein profiles were similar in all the strains and few proteins were differentially expressed during growth as shown by SDS–PAGE. For strain 17, a local strain, a clear relationship was observed between the diminution of immunogenicity and the total loss of protective capacity of sonicated cells at late stationary phase.