Open AccessStructural and serological studies on Hafnia alvei O‐specific polysaccharide of α‐ d ‐mannan type isolated from the lipopolysaccharide of strain PCM 1223
Author(s) -
Katzenellenbogen Ewa,
Kocharova Nina A.,
Zatonsky George V.,
KüblerKiełb Joanna,
Gamian Andrzej,
Shashkov Alexander S.,
Knirel Yurij A.,
Romanowska Elżbieta
Publication year - 2001
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2001.tb01574.x
Subject(s) - microbiology and biotechnology , mannan , strain (injury) , biology , polysaccharide , lipopolysaccharide , serology , hafnia , immunology , antibody , chemistry , biochemistry , anatomy , cubic zirconia , ceramic , organic chemistry
On the basis of chemical and methylation analyses, one‐ and two‐dimensional 1 H‐ and 13 C‐NMR spectroscopy, including COSY, TOCSY, NOESY and 1 H, 13 C HSQC experiments, a neutral O‐specific polysaccharide isolated from Hafnia alvei strain PCM 1223 lipopolysaccharide (LPS) was found to be an α‐mannan composed of pentasaccharide repeating units having the following structure:Immunoblotting showed a strong cross‐reactivity between anti‐ H. alvei PCM 1223 serum and LPSs of Escherichia coli O9 and Klebsiella pneumoniae O3. The serological relationship of the LPSs of these bacteria is due to the structural identity of their O‐specific polysaccharides, though the LPSs differ in their core regions.