Open AccessFlow cytometric analysis of the localization of Helicobacter pylori antigens during different growth phases
Author(s) -
Blom Kristina,
Lundin B.Samuel,
Bölin Ingrid,
Svennerholm AnnMari
Publication year - 2001
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2001.tb01567.x
Subject(s) - helicobacter pylori , antigen , immunoelectron microscopy , biology , flow cytometry , microbiology and biotechnology , hemagglutinin (influenza) , immunofluorescence , virulence , urease , bacteria , spirillaceae , enzyme , antibody , immunology , biochemistry , gastritis , gene , genetics
Previous studies on the localization of several different Helicobacter pylori antigens have been contradictory. We have therefore examined by using both one‐ and two‐color flow cytometry (FCM), immunofluorescence (IF), and immunoelectron microscopy (IEM), the possible surface localization of some H. pylori antigens that may be important virulence factors. All four methods detected the lipopolysaccharide and the N ‐acetyl‐neuroaminyllactose‐binding hemagglutinin protein (HpaA) as surface‐exposed, while the urease enzyme was not detected at all and the neutrophil activating protein only in low concentration on the surface of the H. pylori bacteria during culture of H. pylori in liquid broth for 11 days. The FCM analysis was found to be quite sensitive and specific and also extremely fast compared with IF and IEM, and therefore the preferred method for detection of surface‐localized antigens of H. pylori .