Open AccessSpecific detection of Plesiomonas shigelloides isolated from aquatic environments, animals and human diarrhoeal cases by PCR based on 23S rRNA gene
Author(s) -
GonzálezRey Carlos,
Svenson Stefan B,
Bravo Laura,
Rosinsky Jozef,
Ciznar Ivan,
Krovacek Karel
Publication year - 2000
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2000.tb01512.x
Subject(s) - plesiomonas shigelloides , 23s ribosomal rna , biology , microbiology and biotechnology , polymerase chain reaction , ribosomal rna , diarrhoeal disease , gene , escherichia coli , bacteria , diarrhea , rna , genetics , medicine , ribosome
Twenty‐five strains of Plesiomonas shigelloides isolated from aquatic environment, 10 strains from human cases of diarrhoea and five strains from animals were identified by the polymerase chain reaction technique based on 23S rRNA gene. For this purpose, two primers targeted against part of the 5′ half of the 23S rRNA gene of P. shigelloides ( Escherichia coli number C‐912, G‐1195; Plesiomonas number C‐906, G‐1189) were designed. Results from our study indicated that this method might serve as a tool for a rapid and sensitive identification of P. shigelloides from different environmental and clinical sources.