Open AccessSalmonella enteritidis temperature‐sensitive mutants protect mice against challenge with virulent Salmonella strains of different serotypes
Author(s) -
Gherardi M.Magdalena,
Gómez Marisa I.,
García Verónica E.,
Sordelli Daniel O.,
Cerquetti M.Cristina
Publication year - 2000
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2000.tb01508.x
Subject(s) - salmonella , salmonella enteritidis , biology , microbiology and biotechnology , serotype , heterologous , mutant , virulence , antigen , virology , enterobacteriaceae , bacteria , escherichia coli , gene , genetics
The protection conferred by temperature‐sensitive mutants of Salmonella enteritidis against different wild‐type Salmonella serotypes was investigated. Oral immunization with the single temperature‐sensitive mutant E/1/3 or with a temperature‐sensitive thymine‐requiring double mutant (E/1/3T) conferred: (i) significant protection against the homologous wild‐type Salmonella strains; (ii) significant cross‐protection toward high challenge doses of S. typhimurium . Significant antibody levels against homologous lipopolysaccharide and against homologous and heterologous protein antigens were detected in sera from immunized mice. Moreover, a wide range of protein antigens from different Salmonella O serotypes were recognized by sera from immunized animals. Besides, primed lymphocytes from E/1/3 immunized mice recognized Salmonella antigens from different serotypes. Taken together, these results indicate that temperature‐sensitive mutants of S. enteritidis are good candidates for the construction of live vaccines against Salmonella .