Open AccessEnhancement of IL‐8 production from human monocytic and granulocytic cell lines, THP‐1 and HL‐60, stimulated with Malassezia furfur
Author(s) -
Suzuki Tatsuya,
Tsuzuki Aiko,
Ohno Naohito,
Ohshima Yukio,
Yadomae Toshiro
Publication year - 2000
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2000.tb01471.x
Subject(s) - thp1 cell line , biology , cell culture , monocyte , monocytic leukemia , incubation , cytokine , microbiology and biotechnology , messenger rna , interleukin 6 , immunology , biochemistry , gene , genetics
Previously, we reported that Malassezia furfur , causing systemic fungal infection, was taken up into human monocytic cell line, THP‐1, in a concentration‐dependent manner. This fact suggested that M. furfur could activate phagocytes, such as monocyte and polymorphonuclear leukocyte. Thus we examined cytokine mRNA expression from human monocytic and granulocytic cell line, THP‐1 and HL‐60, stimulated with M. furfur by using reverse transcriptase‐polymerase chain reaction (RT‐PCR) and ELISA. We chose IL‐1α, IL‐6, IL‐8, IL‐12 and TNF‐α as primers for THP‐1, and IL‐1α, IL‐6 and IL‐8 for HL‐60. M. furfur induced the expression of IL‐8 mRNA from THP‐1 and HL‐60 following incubation for 3 h, and also induced IL‐1α mRNA from HL‐60, although this induction was weaker than that of IL‐8 mRNA. Furthermore, opsonized M. furfur induced stronger expression of IL‐8 mRNA in comparison with intact M. furfur . IL‐8 production from THP‐1 and HL‐60 was enhanced in a concentration‐ and incubation time‐dependent manner. These facts strongly suggested that M. furfur could activate phagocytes, and could induce inflammatory responses in systemic infection.