Open AccessThe role of cytotoxic necrotizing factor‐1 in colonization and tissue injury in a murine model of urinary tract infection
Author(s) -
Johnson David E.,
Drachenberg Cinthia,
Lockatell C.Virginia,
Island Michael D.,
Warren John W.,
Donnenberg Michael S.
Publication year - 2000
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.2000.tb01454.x
Subject(s) - microbiology and biotechnology , virulence , escherichia coli , virulence factor , urinary system , cytotoxic t cell , biology , colonization , immunology , in vitro , gene , genetics , endocrinology
Cytotoxic necrotizing factor‐1 (CNF1) is commonly found in Escherichia coli isolates from patients with urinary tract infection (UTI). To determine whether CNF1 is an important UTI virulence factor we compared the ability of a clinical E. coli UTI isolate and a CNF1‐negative mutant of that isolate to colonize and induce histological changes in the urinary tract in a murine model of ascending UTI. We found no evidence that the mutant strain was attenuated.