Open AccessA typical bacterial ornithine‐containing lipid N α ‐( d )‐[3‐(hexadecanoyloxy)hexadecanoyl]‐ornithine is a strong stimulant for macrophages and a useful adjuvant
Author(s) -
Kawai Yohko,
Nakagawa Yoji,
Matuyama Tohei,
Akagawa Kiyoko,
Itagawa Kazutoshi,
Fukase Koichi,
Kusumoto Shoichi,
Nishijima Masahiro,
Yano Ikuya
Publication year - 1999
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1999.tb01718.x
Subject(s) - ornithine , toxoid , chromatography , biochemistry , biology , chemistry , antibody , immunization , amino acid , immunology , arginine
N α ‐[3‐(Hexadecanoyloxy)hexadecanoyl]‐ornithine is a typical bacterial ornithine‐containing lipid (OL). The configuration of the 3‐hydroxy fatty acids in the OL was proved to be d by using HPLC with chiral column. For this analysis, N α ‐( d or l )‐[3‐(hexadecanoyloxy)hexadecanoyl]‐ l ‐ornithine were synthesized and used as standards. The typical bacterial OL, as well as the synthesized one, exhibited strong interleukin‐1‐ and prostaglandin E 2 ‐inducing activities, and further, it induced the production of high IgG anti‐tetanus toxoid antibodies in mice. The typical OL is expected to be utilized as a nontoxic, potent adjuvant.