Open AccessChemotherapy for enterohemorrhagic Escherichia coli O157:H infection in a mouse model
Author(s) -
Yoshimura Kazuaki,
Fujii Jun,
Taniguchi Hatsumi,
Yoshida Shinichi
Publication year - 1999
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1999.tb01376.x
Subject(s) - norfloxacin , fosfomycin , biology , escherichia coli , microbiology and biotechnology , minocycline , antimicrobial , enterobacteriaceae , antibacterial agent , kanamycin , antibiotics , ciprofloxacin , biochemistry , gene
The aim of this study is to evaluate the therapeutic effect of the antimicrobial agents, fosfomycin (FOM), minocycline (MINO), kanamycin (KM) and norfloxacin (NFLX) in the enterohemorrhagic Escherichia coli (EHEC) infected mouse model which we established previously (Infect. Immun. 62 (1994) 3447–3453). Each of the antimicrobial agents, 1/16 LD 50 , was given to the mice per os (p.o.) or intraperitoneally (i.p.) for 3 days after bacterial inoculation and then we observed their mortality rate for 2 weeks. The mortality rates of mice administered with MINO (p.o./i.p.), KM (p.o.), NFLX (p.o./i.p.) were significantly lower than those of the control group. Both the bacterial number and VT2c level in the feces of the FOM group were lower than those of the NFLX group on day 1, but reversed on day 3. In an in vitro experiment, each of the four drugs in combination with mitomycin C (MMC) caused a more significant decrease in the bacterial number than sole MMC, and they consequently indicated the suppressive effect on the release of VT2c.