Open AccessNucleic acid tests for the diagnosis of sexually transmitted diseases 1
Author(s) -
Chernesky Max A
Publication year - 1999
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1999.tb01316.x
Subject(s) - chlamydia trachomatis , nucleic acid , neisseria gonorrhoeae , biology , nucleic acid amplification tests , chlamydia , sexually transmitted disease , nucleic acid thermodynamics , virology , chlamydiaceae , human immunodeficiency virus (hiv) , immunology , microbiology and biotechnology , dna , genetics , syphilis , base sequence
Nucleic acid (NA) assays have been developed and commercialized for many sexually transmitted diseases (STDs). Solid phase, liquid phase or in situ hybridization of nucleic acids without amplification procedures have been successfully used for diagnosing Chlamydia trachomatis, Neisseria gonorrhoeae and human papillomaviruses. Tests which use amplification procedures have provided better sensitivity and specificity than traditional tests. With special temperatures and enzymes, the new tests are designed to amplify either the target nucleic acid or the probe after annealing to the target. A third approach uses signal amplification. This article discusses the technology, specimen requirements and the current status of NA assay performance for diagnosing STDs and HIV by traditional and non‐invasive clinical specimens.