Open AccessInhibition of Candida albicans attachment to extracellular matrix by antibodies which recognize hydrophobic cell wall proteins
Author(s) -
Masuoka James,
Wu Guangqin,
Glee Pati M.,
Hazen Kevin C.
Publication year - 1999
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1999.tb01314.x
Subject(s) - candida albicans , polyclonal antibodies , biology , monoclonal antibody , extracellular matrix , corpus albicans , antiserum , extracellular , germ tube , antibody , microbiology and biotechnology , adhesion , cell adhesion , cell wall , yeast , cell , biochemistry , chemistry , immunology , organic chemistry
Cell surface hydrophobicity influences the adhesive properties of the opportunistic fungal pathogen Candida albicans . Hydrophobic proteins are present in the C. albicans cell wall. These proteins were used to generate a polyclonal antiserum and monoclonal antibodies. We characterized three of these monoclonal antibodies (designated 6C5, 5F8 and 5D8) that recognize different hydrophobic cell wall proteins. Initial characterization of the three antigens, and assessment of their distribution among various Candida species was also carried out. Further, pretreatment of germ tube initials with the mAb inhibits binding of these cells to immobilized extracellular matrix. These results suggest that these hydrophobic proteins are involved in C. albicans adhesion events.