Open AccessProtection against Helicobacter pylori infection by intestinal immunisation with a 50/52‐kDa subunit protein
Author(s) -
Dunkley Margaret L,
Harris Simon J,
McCoy Richard J,
Musicka Melissa J,
Eyers Fiona M,
Beagley Leone G,
Lumley Paul J,
Beagley Kenneth W,
Clancy Robert L
Publication year - 1999
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1999.tb01286.x
Subject(s) - helicobacter pylori , biology , antigen , spirillaceae , microbiology and biotechnology , protein subunit , immunology , virology , immunization , vaccination , gastritis , biochemistry , genetics , gene
A mouse model of Helicobacter pylori infection was used to evaluate the vaccine antigen potential of the citrate synthase homologue protein purified from the H. pylori NCTC 11637 strain. Mice were immunised with the protein by intra‐Peyer's patch immunisation. This route gives maximal intestinal immunisation and was used to screen oral vaccine candidate antigens without the added complication of simultaneously testing oral delivery systems. Two weeks post‐immunisation mice were infected with Sydney strain H. pylori and 4 weeks after infection the mice were killed and the level of H. pylori infection in the stomach determined. Pre‐immunisation with the 50/52‐kDa protein led to a 84–91% reduction in H. pylori infection compared to unimmunised controls.