Open AccessImmunological evaluation of novel Mycobacterium tuberculosis culture filtrate proteins 1
Author(s) -
Weldingh Karin,
Andersen Peter
Publication year - 1999
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1999.tb01235.x
Subject(s) - tuberculosis , mycobacterium tuberculosis , biology , microbiology and biotechnology , antigen , immunity , interferon gamma , virology , tuberculosis vaccines , immunology , mycobacterium , tuberculosis diagnosis , mycobacterium bovis , bcg vaccine , bacteria , immune system , vaccination , medicine , genetics , pathology
Culture filtrate from Mycobacterium tuberculosis contains molecules which can promote protective immunity to tuberculosis in animal models. Six novel proteins in the region of 17–29 kDa were purified and investigated for their immunological relevance in M. tuberculosis ‐infected mice, guinea pigs and tuberculosis patients. The proteins CFP17, CFP21, CFP25 and CFP29 were all identified as strong interferon‐γ inducers in M. tuberculosis ‐infected mice and in tuberculosis patients. The CFP21 protein is encoded in the genomic region RD‐2 which is deleted from a number of BCG strains and the diagnostic potential of this antigen was evaluated.