Open AccessIdentification of S, F1C and three PapG fimbrial adhesins in uropathogenic Escherichia coli by polymerase chain reaction
Author(s) -
Mitsumori Kenji,
Terai Akito,
Yamamoto Shingo,
Yoshida Osamu
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01173.x
Subject(s) - bacterial adhesin , biology , microbiology and biotechnology , escherichia coli , fimbria , polymerase chain reaction , virulence , virulence factor , enterobacteriaceae , gene , genetics
S and F1C fimbrial adhesins often expressed by uropathogenic Escherichia coli are genetically homologous. A multiply primed polymerase chain reaction (PCR) was developed for discriminating the S ( sfa ) and F1C ( foc ) fimbrial operons. A total of 270 uropathogenic E. coli strains and 80 fecal isolates were examined. PCR specifically detected the sfa and foc alleles in 105 (93%) of 113 sfa / foc + strains by DNA hybridization. Furthermore, 87% of sfa + uropathogenic E. coli simultaneously possessed the genes encoding the class III P fimbrial adhesin ( prsG J96 ), α‐hemolysin and cytotoxic necrotizing factor 1. Statistical analysis showed the class II P fimbrial adhesin ( papG IA2 ) and F1C fimbria to be associated with high relative virulence in pyelonephritis and cystitis, respectively. The multiply primed PCR developed should be useful for assessing the contribution of the S and F1C fimbriae in the pathogenesis of urinary tract infections.