Open AccessImmunological and chemical studies of Salmonella haarlem somatic antigen epitopes. II. Serological investigations
Author(s) -
Dziadziuszko Halina,
Kunikowska Danuta,
Głośnicka Renata,
Gajdus Jerzy,
Kaczyński Zbigniew,
Szafranek Janusz
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01172.x
Subject(s) - epitope , antiserum , antigen , polyclonal antibodies , hemagglutination , microbiology and biotechnology , biology , salmonella typhi , monoclonal antibody , antigenicity , sodium borohydride , bacteria , chemistry , antibody , biochemistry , escherichia coli , catalysis , immunology , gene , genetics
Lipopolysaccharide of Salmonella haarlem was hydrolyzed and the products separated. Native O‐polysaccharide antigen was oxidised with sodium periodate followed by reduction with sodium borohydride. Native and chemically modified antigens were the subject of immunochemical studies. Monoclonal antibodies against S. haarlem and polyclonal rabbit antisera against S. haarlem , S. typhi and S. anatum bacteria were produced. The serological relationship between the lipopolysaccharide of Salmonella bacteria belonging to the two different groups D 2 and E 1 was demonstrated using haemagglutination reactions, inhibition of haemagglutination and immunoblotting. Cross‐reactions were observed in haemagglutination reactions and in immunoblotting between antisera to S. haarlem , S. typhi and S. anatum . Factors 3,9,46 were found in the S. haarlem strain and the sugar composition for the epitopes of each factor was determined.