Open AccessImmunological and chemical studies of Salmonella haarlem somatic antigen epitopes. I. Structural studies of O‐antigen
Author(s) -
Szafranek Janusz,
Gajdus Jerzy,
Kaczyński Zbigniew,
Dziadziuszko Halina,
Kunikowska Danuta,
Głośnicka Renata,
Yoshida Takashi,
Vihanto Jouko,
Pihlaja Kalevi
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01171.x
Subject(s) - heteronuclear molecule , anomer , antigen , epitope , biology , rhamnose , galactose , microbiology and biotechnology , stereochemistry , h antigen , somatic antigen , mannose , nuclear magnetic resonance spectroscopy , biochemistry , chemistry , immunology
Lipopolysaccharide (LPS) of Salmonella haarlem was hydrolyzed and the products separated. The structure of the O‐specific polysaccharide (OPS) was found from sugar and methylation analyses. Rhamnose, mannose, galactose and tyvelose were detected and their linkage modes were established. The structure was confirmed by 1 H, homonuclear and heteronuclear correlations and 13 C NMR spectra. Anomeric configurations were assigned by chromium trioxide oxidation and proton coupled 13 C spectra. Sugar sequence was established from specific carbon shift data and nuclear Overhauser effect spectroscopy. The repeating unit structure of S. haarlem OPS as →3)‐α‐ d ‐Galp‐(1→6)‐[α‐Tyvp‐(1→3)]‐β‐ d ‐Manp‐(1→4)‐α‐ l ‐Rhap was estimated. No structural heterogeneity of the antigen was found.