Open AccessBiosensor characterization of antigenic site A of foot‐and‐mouth disease virus presented in different vector systems
Author(s) -
Benito Antoni,
Regenmortel Marc H.V
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01155.x
Subject(s) - foot and mouth disease virus , biology , virology , vector (molecular biology) , biosensor , foot and mouth disease , antigen , virus , immunology , recombinant dna , biochemistry , gene
The region 141–160 of the VP1 protein of foot‐and‐mouth disease virus known as site A is an immunodominant region that has been extensively studied for the purpose of developing a synthetic vaccine. In the present study, site A of foot‐and‐mouth disease virus was inserted in three different loops of the maltose‐binding protein and its antigenicity was compared with site A presented as a conjugated synthetic peptide or inserted in β‐galactosidase. The affinity of antibodies elicited against the site A synthetic peptide was also compared with that of antibodies raised against the site A inserted within the two carrier proteins. Using biosensor technology it was possible to estimate the concentration of site A antibodies present in the various antisera and to show that site A fused to maltose‐binding protein was a slightly better mimic of the epitope present in the virus particle than the synthetic peptide or the β‐galactosidase recombinant construct.