Open AccessStructure of the O‐specific polysaccharide of Proteus penneri strain 41 from a new proposed serogroup O62
Author(s) -
Zych Krystyna,
Knirel Yuriy A,
Paramonov Nikolay A,
Vinogradov Evgeny V,
Arbatsky Nikolay P,
Senchenkova Sofya N,
Shashkov Alexander S,
Sidorczyk Zygmunt
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01143.x
Subject(s) - heteronuclear molecule , strain (injury) , proteus , polysaccharide , nuclear magnetic resonance spectroscopy , biology , microbiology and biotechnology , acetylation , stereochemistry , chemistry , biochemistry , escherichia coli , anatomy , gene
O‐specific polysaccharide of Proteus penneri strain 41 was studied using 1 H‐ and 13 C‐NMR spectroscopy, including two‐dimensional COSY, heteronuclear 13 C, 1 H‐correlation (HETCOR) and one‐dimensional NOE spectroscopy, and the following structure of a non‐stoichiometrically O ‐acetylated hexasaccharide repeating unit was established:where RGlcNAc is 2‐acetamido‐4‐ O ‐[( S )‐1‐carboxyethyl]‐2‐deoxyglucose. Cross‐reactivity of anti‐ P. penneri 41 O‐serum with other P. penneri strains is discussed, and a new, separate O62 serogroup is proposed which is the next Proteus O‐serogroup containing P. penneri strains only.