Open AccessSuppression of macrophage activation by peritoneal fluid from patients with endometriosis
Author(s) -
Takeshita Shigeki,
Dobashi Kazuyoshi,
Abe Shigeru,
Tansho Shigeru,
Yamaguchi Hideyo,
Okinaga Shoichi,
Mori Hiroyuki
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01133.x
Subject(s) - peritoneal fluid , endometriosis , tumor necrosis factor alpha , peritoneum , peritoneal cavity , macrophage , cytokine , biology , pathology , medicine , immunology , in vitro , anatomy , biochemistry
To investigate the effects of peritoneal fluid from patients with endometriosis on mouse peritoneal macrophages (Mφ), peritoneal fluid from endometriosis patients ( n =15 ) were added to a monolayer of C3H/HeJ mouse peritoneal Mφ. Tumor necrosis factor‐producing activity was measured by the L929 assay activated with FK‐23 (a preparation of heat‐killed Enterococcus faecalis ). Tumor necrosis factor‐producing activity of C3H/HeJ mouse peritoneal Mφ incubated with peritoneal fluid was suppressed in 14 endometriosis patients. Interestingly, in nine endometriosis patients, tumor necrosis factor‐producing activity was much lower than seen with mouse peritoneal Mφ incubated with corticosterone. Peritoneal fluid contains suppressive properties for the activation of peritoneal Mφ, which might allow the implantation of free endometrial cells or the metaplastic phenomena stimulated by retrograde menstruation.