Open AccessMorphological effects of itraconazole on murine macrophage
Author(s) -
Ochi Hisako,
Ishijima Sanae A,
Abe Shigeru,
Tameike Akane,
Yamaguchi Hideyo,
Osumi Masako
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01126.x
Subject(s) - itraconazole , ketoconazole , biology , macrophage , microbiology and biotechnology , in vitro , biochemistry , antifungal
Morphological effects of itraconazole (ITCZ) on murine macrophages were examined by light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and compared with the effects of other antifungal agents. Thioglycolate‐induced peritoneal macrophages were prepared from C3H/He J mice and cultured for 20 h in the presence of the antifungal azoles econazole, fluconazole, miconazole, ketoconazole, ITCZ, hydroxy‐itraconazole (ITCZ‐OH), and a polyene antibiotic amphotericin B (AMPH). Among these reagents, only ITCZ and its derivative ITCZ‐OH were effective in causing morphological changes of murine macrophage as determined by LM and SEM. Macrophages treated with 2 μg/ml ITCZ or ITCZ‐OH were stretched out bidirectionally, their surface was smooth and their ‘ruffles’ decreased. TEM observation showed that the bundles of the filamentous structure existed along the cell shape in the cytoplasm. These findings suggest that ITCZ and ITCZ‐OH affect the morphology of macrophages.