Open AccessEffect of Pasteurella haemolytica outer membrane proteins on bovine neutrophils
Author(s) -
Iovane Giuseppe,
Galdiero Massimiliano,
Vitiello Mariateresa,
Martino Luisa
Publication year - 1998
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1998.tb01108.x
Subject(s) - microbiology and biotechnology , biology , bacterial outer membrane , pasteurella , bacteria , bacterial protein , membrane protein , pasteurellosis , pasteurella multocida , escherichia coli , membrane , biochemistry , genetics , gene
The major outer membrane proteins (OMPs) isolated from Pasteurella haemolytica induce alterations of the biological activity of bovine polymorphonuclear leukocytes (PMNs). A dose‐dependent reduction of the capacity of adherence to nylon wool in vitro was observed. OMPs also acted as chemotaxins at concentrations between 5 and 20 μg/ml. Concentrations lower than 5 μg/ml did not give considerable results. Preincubation with 5 μg/ml of OMPs led to modifications in the values of the phagocytic index and of intracellular killing, which were found to be diminished with respect to controls.