Open AccessMycoplasma salivarium induces interleukin‐6 and interleukin‐8 in human gingival fibroblasts
Author(s) -
Shibata Kenichiro,
Hasebe Akira,
Sasaki Tsuguo,
Watanabe Tsuguo
Publication year - 1997
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1997.tb01097.x
Subject(s) - biology , interleukin , fibroblast , microbiology and biotechnology , intracellular , cytokine , interleukin 15 , cell culture , mycoplasma , immunology , genetics
Analysis by an enzyme‐linked immunosorbent assay for cytokines indicated that whole cells, intracellular materials and cell membranes of Mycoplasma salivarium induced interleukin‐6 and interleukin‐8 in a human gingival fibroblast cell line, Gin‐1 cells. This was confirmed by reverse transcription‐polymerase chain reaction analysis of mRNAs of these cytokines. Studies with inhibitors of second‐messenger pathway indicated that a protein kinase C‐dependent pathway was involved in the expression of the activity of the cell membranes. In addition, whole cells of other mycoplasmas ( M. hominis , M. arthritidis , M. arginini , M. fermentans , M. penetrans , M. pirum and M. pneumoniae ) tested for comparative purposes were also shown to possess the activity. Thus, this study demonstrated that mycoplasmas possess the activity to induce interleukin‐6 and interleukin‐8 in human fibroblasts.