Open AccessGenetic typing methods applied to the differentiation of clonal lines among Salmonella enterica serogroup G strains causing human salmonellosis
Author(s) -
Martín M.C.,
GonzálezHevia M.A.,
Moro I,
Mendoza M.C.
Publication year - 1997
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1997.tb01090.x
Subject(s) - ribotyping , typing , biology , salmonella , salmonella enterica , outbreak , rapd , serotype , microbiology and biotechnology , restriction fragment length polymorphism , virology , genetics , genotype , gene , medicine , genetic diversity , bacteria , population , environmental health
In Spain, in November 1995, an epidemiological alert recommended the surveillance of Salmonella serogroup G. The nine clinical isolates collected after and the four collected before the alert in Asturias were differentiated into six clonal lines by the combination of results from Hin cII ribotyping, PCR ribotyping, and RAPD typing using primers named A and S. The seven Gumpensis isolates showed identical DNA fingerprinting with the four typing procedures falling into a line. Six of these were collected during May–August from people living in a single health area suggesting that they could be associated with a community outbreak. The four Worthington isolates fell into three other lines, one Poona isolate into another line and one Havana isolate into another. 100% typeability was shown with all methods. The reproducibility of Hin cII ribotyping was better than that of PCR‐based methods, although these were less time‐consuming. The highest discriminatory power was obtained with Hin cII ribotyping and RAPD typing using primer A.