Open AccessContribution of neutrophils to lipopolysaccharide‐induced tumor necrosis factor production and mortality in a carrageenan‐pretreated mouse model
Author(s) -
Matsumoto Takahiro,
Yoshida Shinichi,
Shiga Yosuke,
Kikuchi Makoto,
Sata Takeyoshi,
Shigematsu Akio
Publication year - 1997
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1997.tb01010.x
Subject(s) - lipopolysaccharide , tumor necrosis factor alpha , immunology , carrageenan , biology , cyclophosphamide , in vitro , necrosis , tumor necrosis factor α , microbiology and biotechnology , pharmacology , chemotherapy , biochemistry , genetics
Carrageenan (CAR) pretreatment primes mice for lipopolysaccharide (LPS)‐induced tumor necrosis factor (TNF)‐ α production in sera and increases their mortality rate. To study the contribution of neutrophils in this model, blood neutrophil count was regulated with cyclophosphamide. After LPS challenge, both serum TNF‐ α activity and mortality risk ratio were significantly higher in neutrophilic mice, but significantly lower in neutropenic mice. In vitro, CAR treatment primed for TNF‐ α production of blood neutrophils, but conversely, that of monocytes was suppressed. We suggest that neutrophils are the major cells to produce TNF‐ α and to determine mouse mortality after LPS challenge in the mouse CAR model.