Open AccessA purified protein from Salmonella typhimurium inhibits high‐affinity interleukin‐2 receptor expression on CTLL‐2 cells
Author(s) -
Arai Toshihiko,
Matsui Katsuhiko
Publication year - 1997
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1997.tb01008.x
Subject(s) - interleukin 2 , internalization , biology , receptor , cell culture , cytotoxic t cell , cell growth , t cell , microbiology and biotechnology , salmonella , receptor expression , immune system , biochemistry , immunology , in vitro , bacteria , genetics
Previously, we have demonstrated that the immunosuppression induced by the purified substance Salmonella typhimurium ‐derived inhibitor of T‐cell proliferation (STI) involves T‐cell non‐responsiveness to interleukin‐2 (IL‐2). In the present study, it was found that STI inhibited the growth of CTLL‐2 cells, which are an IL‐2‐dependent cytotoxic T‐cell line. Analysis of IL‐2 receptor (IL‐2R) function showed that STI inhibited high‐affinity receptor expression and internalization by CTLL‐2 cells. Furthermore, FACS analysis demonstrated that STI inhibited both β chain and γ chain expression of IL‐2R on the cells. These results suggest that the suppression of T‐cell proliferation induced by STI results from a defect in IL‐2R function.