Open AccessEffect of Escherichia coli L‐form cytoplasmic membranes on the interaction between macrophages and Lewis lung carcinoma cells: scanning electron microscopy
Author(s) -
Ivanova E.H.,
Michailova L,
Stefanova Z,
Neychev H,
Radoevska S,
Gumpert J
Publication year - 1997
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1997.tb00993.x
Subject(s) - lewis lung carcinoma , phagocytosis , macrophage , in vitro , biology , pseudopodia , cytoplasm , scanning electron microscope , microbiology and biotechnology , membrane , escherichia coli , lipopolysaccharide , electron microscope , cell , immunology , materials science , biochemistry , metastasis , cancer , genetics , physics , optics , gene , composite material
Scanning electron microscopy (SEM) investigations on the interactions between peritoneal macrophages from Lewis lung carcinoma (LLC)‐bearing mice and LLC tumour cells during 21 days after tumour implantation were carried out. The action of lipopolysaccharide (LPS)‐containing cytoplasmic membranes (CM), from the stable protoplast type L‐form of Escherichia coli , on the activity of in vitro phagocytosis was studied; CM induced a continuous increase in macrophage numbers. Activation of macrophage surfaces in healthy and tumour‐bearing mice was established. Lamelipods, pseudopods and migration fringes 14 days after CM application were seen. Crater‐like cavities deeply in the macrophage cells as well as adherent or prominent engulfed tumour cells within macrophages were observed during in vitro interaction with LLC cells. Macrophages from tumour‐bearing mice without CM treatment showed less activation evaluated by SEM during earlier stages of tumour growth. The SEM investigation proved the temporary stimulating effect of E. coli L‐form CM on the cell surface activation of peritoneal macrophages in healthy and LLC‐bearing mice.