Open AccessRapid and differential detection of two analogous enterotoxins of Vibrio cholerae and enterotoxigenic Escherichia coli by a modified enzyme‐linked immunosorbent assay
Author(s) -
Koike Norio,
Okada Keishi,
Yabushita Yasunori,
Zhang DongYan,
Yamamoto Koichiro,
Miwatani Toshio,
Honda Takeshi
Publication year - 1997
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1997.tb00992.x
Subject(s) - vibrio cholerae , enterotoxin , microbiology and biotechnology , cholera toxin , biology , enterotoxigenic escherichia coli , escherichia coli , vibrionaceae , toxin , virology , bacteria , biochemistry , gene , genetics
The principle of a novel ELISA (nylon‐slip immuno‐test, NSIT) was applied to the differential detection of two analogous enterotoxins, cholera toxin (CT) of Vibrio cholerae and heat‐labile enterotoxin (LT) of enterotoxigenic Escherichia coli . The results obtained for CT and LT detection by a single test were sufficiently sensitive (87.9 and 100%) and specific (100 and 94.7%) in the differential detection test, when compared with the result of a colony hybridization test with DNA probes. The results suggest that the novel ELISA is applicable to the diagnosis of bacterial infections, by means of differential immunological detection of toxins in a single test.