Open AccessLysophospholipase L 2 of Vibrio cholerae O1 affects cholera toxin production
Author(s) -
Whayeb Shatha A.,
Yamamoto Koichiro,
Castillo MeliaEllen Y.,
Tojo Hiromasa,
Honda Takeshi
Publication year - 1996
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1996.tb00352.x
Subject(s) - vibrio cholerae , lysophospholipase , cholera toxin , biology , microbiology and biotechnology , mutant , hemolysin , plasmid , vibrionaceae , el tor , toxin , gene , cholera , bacteria , virulence , genetics , biochemistry , enzyme , phospholipase
The implication in cholera toxin (CT) production of the newly identified gene, lypA , that encodes the lysophospholipase L 2 of Vibrio cholerae , was investigated. Introduction of lypA into the V. cholerae O1 mutant (NF404), which has a Tn5‐insertion in lypA and has lost CT as well as haemolysin production, restored the lysophospholipase activity and CT production but not the haemolytic activity. Inactivation of the lypA gene of the wild‐type strain by chromosomal integration of a plasmid containing a portion of the lypA gene decreased the lysophospholipase L 2 activity and the production of CT but not the haemolytic activity. Furthermore, constructed mutants of El Tor‐biotype and Classical‐biotype strains which have a defective lypA failed to produce CT and exhibited decreased enterotoxicity in the ligated rabbit ileal loop test. These results suggest that lypA is possibly required for the expression of CT and may play a role in pathogenicity of V. cholerae .