Open AccessComparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus‐PCR for epidemiological studies of Salmonella
Author(s) -
Millemann Yves,
LesageDescauses MarieClaude,
Lafont JeanPierre,
ChaslusDancla Elisabeth
Publication year - 1996
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1996.tb00279.x
Subject(s) - rapd , ribotyping , biology , intergenic region , salmonella , salmonella enteritidis , typing , genetics , polymerase chain reaction , dna profiling , microbiology and biotechnology , dna , gene , bacteria , genome , population , genetic diversity , demography , sociology
Discrimination of 70 Salmonella strains previously studied by ribotyping was realized by RAPD and ERIC‐PCR analysis. RAPD results on the 56 S. typhimurium isolates did not closely match those of ribotyping. With ERIC‐PCR, two fingerprints only were obtained. For the 14 S. enteritidis strains, a helpful discrimination was obtained with RAPD analysis, while ERIC‐PCR resulted in a single fingerprint.